The Effect of Water Temperature and Alcohol Concentration on Membrane Permeability Free essay! Download now
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The Effect of Water Temperature and Alcohol Concentration on Membrane Permeability
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| Words: 900 | Submitted: 06-Jun-2010
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DescriptionThe Effect of Water Temperature and Alcohol Concentration on Membrane Permeability
Method A fresh cylinder of beetroot was taken and using a scalpel any
skin was removed. Care should be taken when a scalpel is used to cut
objects into small pieces. It was then cut into 40 discs using the
scalpel. Next the discs were placed in a boiling tube and rinsed
repeatedly in till the water ran clear. Then two sets of five boiling
tubes were set up into racks. Then using a measuring cylinder measured
10cm³ of tap water was placed in five of the boiling tubes of the set
that would be testing the effect of different temperatures. These were
then left in the different water baths to adjust to the temperature.
The other five had 10cm³ different concentrations of alcohol ranging
from 25%-100%. There was also one with 10cm³ of water. Next four discs
of the beetroot were placed into each boiling tube. They were then
left for about 10 minutes. While this was going on the colorimeter was
set up and adjusted with water ‘blank’ to 100% transmission using a
blue/green filter. When each sample had been left for a sufficient
amount of time some of the bathing liquid was removed from each
beetroot sample. It was then placed in the colorimeter. Readings were
then taken and recorded.
7 80 20 92 40 94 60 19 87 12
0 88 25 54 50 38 75 34 100 24
Conclusion The experiment showed that different temperatures of water
had different effects on the permeability of the cell membranes. This
was because as the water got heats up it damages the membranes. Using
too much heat denatures the proteins, which alters the properties and
can close the channels. It cannot be reversed back.
In my results it shows that as the temperature rises to a certain
point the transmission increases and then after 40°C the cell membrane
starts to be more damaged.
In my graph it shows that at 7°C it still has quite a high
transmission percentage at 80%. It continues to increase slowly till
it gets above 40°C and then it drops from 94% to 19% at 60°C. It goes
down to 12% at 87°C.
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