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Plant tissue culture
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| Words: 1800 | Submitted: 12-May-2009
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DescriptionPurpose: To demonstrate the totipotency of plant cells.
Importantly, totipotent cells must be able to differentiate not only into any cell in the organism, but also into the extraembryonic tissue associated with that organism. For example, human stem cells are considered totipotent only if they can develop into any cell in the body. Unlike animal cells, most plant cells remain totipotent throughout the life of the plant. Differentiation is irreversible in animal cells but many differentiated in plant cells, therefore it can develop into a complete new plant as they have ability to regenerate identical whole plant clone from root, stem or leaf cuttings.
Hence, totipotency of plant cells allows plants to be reproduced using plant tissue culture. Plant tissue culture is a practice used to propagate plants under sterile conditions, often to produce clones of a plant. Explants are one of example which sterilized the small pieces of plants and then placed on a solid agar medium to allow it to grow. Plant tissue culture may offer certain advantages over traditional methods of propagation: (1) the production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits. (2) The regeneration of whole plants from plant cells that have been genetically modified. (3) The production of plants from seeds that otherwise have very low chances of germinating and growing. (4) The production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens. (5) To quickly produce mature plants. Also, Plant tissue culture is so important in plant biology research, plant breeding, genetic modification of plants and in the conservation of endangered plants.
I think all the explants will grow healthy with a new stem, new leaves but it will stop to grow later on as the agar provides nutrients and other needs for them, however the nutrients are limited from agar.
The controlled of plant tissue culture are light and temperature. Light and temperature are the main factors for photosynthesis, therefore plants can be growth. However the compounding of it is nutrients. As we cant control how much nutrients can be provided by agars to the explants. Different volume or size of agars may contain different quantity of nutrients.
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