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Catalase in potato and hydrogen peroxide concentration Free essay! Download now

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Catalase in potato and hydrogen peroxide concentration

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Downloads to date: N/A | Words: 1040 | Submitted: 10-May-2011
Spelling accuracy: 97.4% | Number of pages: 6 | Filetype: Word .doc


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Catalase in potato and hydrogen peroxide concentration

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Catalase in potato and hydrogen peroxide concentration

Introduction
Hydrogen peroxide (H2O2) is a by-product of respiration and is made in all living cells. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. Cells make the enzyme catalase to remove hydrogen peroxide. This investigation looks at the rate of oxygen production by the catalase in pureed potato as the concentration of hydrogen peroxide varies.

The reaction of catalase in the decomposition of hydrogen peroxide is:
2 H2O2 ? 2 H2O + O2












Catalase is used in the food industry for removing hydrogen peroxide from milk prior to cheese production. Another use is in food wrappers where it prevents food from oxidizing. Catalase is also used in the textile industry, removing hydrogen peroxide from fabrics to make sure the material is peroxide-free.




A minor use is in contact lens hygiene - a few lens - cleaning products disinfect the lens using a hydrogen peroxide solution; a solution containing catalase is then used to decompose the hydrogen peroxide before the lens is used again. Recently, catalase has also begun to be used in the aesthetics industry. Several mask treatments combine the enzyme with hydrogen peroxide on the face with the intent of increasing cellular oxygenation in the upper layers of the epidermis.










Aim: To investigate the effect of Catalase on H2O2 and the volume of O2 produced.

Hypothesis: As the concentration of Catalase increases from 20.25 cm to 22 cm potato cylinders, the volume of O2 will also increase approximately twice.


Variables:
Dependant: Volume of O2
Independent: Temperature of environment
Controlled: volume of H2O2, mass or volume of disk potatoes, concentration of H2O2, volume of water in the collector.

Apparatus & Chemicals:
Hydrogen peroxide, 20 vol
Potato Cylinders, fresh, 20.25 cm, 20.50 cm, 21.00 cm, 22.00 cm
Rubber bung, 1-holed, to fit test tube delivery tube in the hole (connected to 50 cm rubber tubing)
Access to suitable sink of water
4 Test tubes
2?Syringe (5 cm3)
1?Measuring cylinder, 50 cm3
2?Stopclock/ stopwatch

Health & Safety
Wear eye protection and cover clothing when handling hydrogen peroxide. Wash splashes of pureed potato or peroxide off the skin immediately. Be aware of pressure building up if reaction vessels become blocked. Take care inserting the bung in the conical flask it needs to be a tight fit, so push and twist the bung in with care.
SAFETY: Wear eye protection and protect clothing from hydrogen peroxide. Rinse splashes of peroxide and pureed potato off the skin as quickly as possible.
The tube will get hot during the reaction. So be aware.

Method:

We put the potato cylinders inside the test tubes marked showing the length of the cylinders putted inside them.
Then we prepare the measuring column as shown below.


Before putting the bung on the tube, we insert 5 cm3 of H2O2 ...

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